PACAP antibody (C-Term)

Catalog No. ABIN571003

This Goat Polyclonal antibody specifically detects PACAP in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PACAP antibody (C-Term) (ABIN571003)

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PACAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PACAP Antibody

Binding Specificity: C-Term

Purpose: PACAP

Sequence: GKRYKQRVKN KGRR

Specificity: Reported variants represent identical protein: NP_001093203.1, NP_001108.2.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GKRYKQRVKNKGRR, from the C Terminus of the protein sequence according to NP_001108.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25 kDa band observed in Human Brain (Cerebral Cortex, Cerebellum and Hippocampus) lysates (calculated MW of 18.8 kDa according to NP_001108.2). This molecular weight is routinely observed by other sources. Recommended concentration: 1-

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PACAP

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Alternative Name: ADCYAP1

Background: ADCYAP1, adenylate cyclase activating polypeptide 1 (pituitary), MGC126852, PACAP, OTTHUMP00000162201, adenylate cyclase activating polypeptide

Gene ID: 116, 11516, 24166

NCBI Accession: NP_001108

Pathways: Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling